The recent COVID19 outbreak has greatly affected public health labs' supply chains, sample collection, and transportation of samples. According to a report by National Public Radio, this may be partially responsible for a decrease in new viral tests. Because of the limited availability of VTM, the US government commissioned the GEIS to perform a bridge study in which saline and RNAlater were evaluated as alternatives.
To determine the stability of SARS-CoV-2 RNA, several methods were used. PCR was performed on four common viruses and found to be reliable in detecting virus and pathogen RNA after long storage. These methods have improved the accuracy of SARS-CoV-2 diagnosis by more than five times. A high-titer remnant of SARS-CoV-2 was spiked into pooled SARS-CoV-2 RNA-negative specimen remnants.
However, the stability of SARS-CoV-2 RNA is unknown. In vitro studies of SARS-CoV-2 RNA, there are no reliable methods to detect SARS-CoV-2 RNA in samples stored at four different temperatures. This is due to a lack of stability of viral RNA in vitro. A recent study conducted by Druce et al. (33) has demonstrated that SARS-CoV-2 RNA can be reliably amplified even after long storage in cold storage.
However, SARS-CoV-2 RNA stability has been studied and compared with other commonly used viruses. In this study, they found that SARS-CoV-2 RNA was stable at 4degC. This is a significant improvement over existing methods, which require a lower temperature. The findings suggest that SARS-CoV-2 RNA can be reliably amplified after long storage. Its stability can be used in clinical settings as well.
The stability of SARS-CoV-2 RNA was determined by Druce et al. using a pool of patient sputum, and RNA from influenza virus. These studies found that SARS-CoV-2 RNA was stable in these samples for up to 24h in cold storage. The findings also showed that SARS-CoV-2 RNA can be reliably amplified after a long storage time.
Unlike traditional methods of sample collection, the DNA/RNA Shield is portable and compatible with a variety of specimens. The system is a convenient, sterile plastic tube, and is approved for long-term freeze-stable storage of clinical specimens containing viruses. The system allows researchers to work in the field and travel internationally, without the risk of infection and retain the viability of the organism for up to 48 hours.
The COPAN resource page contains COVID-19 guidelines and distributor part numbers. The UTM has been cleared for use in the United States by the FDA. The UTM is a sterile, screw-cap collection system that can be used for both transporting and preserving clinical specimens. Its sterility is an added benefit, as it can preserve the organism for up to 48 hours. Its ability to maintain viability in cold temperatures is critical for the successful use of the virus transport media.
Puritan viral transport media maintains the viability of clinically relevant viruses in laboratory tests. The BD and Puritan medical products companies offer several types of these transport media to fit various needs and budgets. These are available in different strengths, including those for culture and isolation, and can be a valuable asset to any laboratory. The Puritan line of products is available in both liquid and solid forms. Each of these products offers different features, making them ideal for different applications.
Opti-Swab and UniTranz transport systems are economical and versatile for multiple samples. The Opti-Swab system is a great option for Bordetella pertussis culture. The medium is stable at room temperature and leak-proof. The swab tip is made from spun polyester and is compatible with the Puritan UniTranz-RT system. Both kits contain PurFlock Ultra swabs. Both types of swabs are available in different sizes and tip shapes, making them easier to use. The spun polyester swabs are manufactured in the United States.
Puritan's UniTranz transportation system is versatile and leak-proof. Its unique cap design prevents the swab from spilling. The UniTranz-RT is compatible with all types of culture media, and its versatility makes it a great choice for laboratories of all sizes. Its self-centering cap facilitates easy swab removal, and the Opti-Swab is also convenient. Its unique design makes it a good choice for testing small specimens because it can preserve the viability of aerobic and anaerobic bacteria and is suitable for high-throughput applications.
Puritan UniTranz-RT is a versatile and leak-proof transport system. It is especially useful for the detection of Bordetella pertussis using real-time Q-PCR. The Opti-Swab kit contains a PurFlock Ultra swab. Its Modified Opti-Swab is a high-quality and reliable system for testing Bordetella pertussis and other viruses.
Puritan UniTranz-RT is a universal transport medium that can be used for many applications. It is highly versatile and stable at room temperature. Its cap is leak-proof and makes it easy to remove the swab without spilling the medium. It is also designed for fastidious and aerobic bacteria. The Opti-Swab is particularly efficient for smaller specimens. It is also suitable for culture of large amounts of DNA.
The UniTranz-RT is a popular universal transport medium that is used to collect and analyze samples from human respiratory specimens. It is suitable for detecting virus, chlamydia, mycoplasma, and ureaplasma. The company has been manufacturing this product for over 60 years. Its products have many applications in the laboratory and are widely available. It is the perfect choice for labs.
The PurSafe DNA/RNA transport medium is suitable for ambient storage. It also ensures safe transportation and inactivation of infectious agents. The UniTranz-RT 3ml Mini-Tip Transport System is a highly efficient way to capture more cells. The PurFlock Ultra is the purest version of the PurFlock. It is also a popular option for rapid diagnostic tests. Its high purity makes it ideal for various laboratory applications.